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A: Check if you still have the file located in your user’s AppData\Local\username\ folder. If so, delete it and rename it to the same name without an extension. The long-term goal of this application is to study the physiology and pharmacology of inhibitory descending serotonergic (5-HT) pathways in the ventral medulla oblongata (VMO), with an emphasis on the role of 5-HT1A autoreceptors. This neural system is anatomically distinct from the dorsal and median raphe nuclei, and provides anatomical and physiological evidence for an important modulatory role for 5-HT in regulating hypothalamic-pituitary-adrenal (HPA) axis activity. Not only do HPA responses to stress decrease in animals treated with drugs that attenuate 5-HT function, there is also an intrinsic inhibitory tone at the level of the VMO. This tonic activity is mediated by 5-HT1A autoreceptors, and is antagonized by pharmacological agents that block 5-HT1A receptors. The proposed research plan seeks to elucidate the physiological role of this inhibitory system, and to determine the mechanisms of its modulation by 5-HT1A autoreceptors. These studies will employ a series of electrophysiological, pharmacological, and molecular approaches in vitro. Specific aim 1 will establish the chemical identity and distribution of 5-HT1A autoreceptors in the VMO, and will characterize their ability to modulate the intrinsic rate of excitation of medullary neurons. Experiments will utilize immunocytochemical and molecular approaches to identify the 5-HT1A receptor subtype(s) present in the VMO. The experiments will also determine whether neuronal activity in the VMO is affected by 5-HT1A autoreceptor-mediated inhibition, and whether the inhibitory tone in the VMO arises from a pre- or postsynaptic locus. Specific aim 2 will determine whether the activation of 5-HT1A autoreceptors alters HPA output in vivo, i.e., alters the magnitude and/or duration of the stress response, and whether this response is mediated by activation of GABA-GABAB or GABAA receptors. The experiments will make use of a series of GABA receptor-selective drugs, coupled with measurements of HPA activity. Specific aim 3 will determine whether inhibition of 5-HT1A autoreceptors

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. By bassamitvi.Egmont (painting) Egmont (also Egmont-Bund, Catalan: Egó) (c.1795–1860) was a Flemish Romantic painter known for landscapes and architecture in oils and watercolours. Born in Antwerp, he spent much of his career in London (with a few years in Paris and Milan). He is sometimes considered the first Belgian painter. His father, also named Egmont (1671–1743), was a portrait painter who married his mother, Elise Ligard, in 1690. Egmont’s first recorded sale was of a Claude Lorrain watercolour in 1810. He had some training in Antwerp, where he was successful with painting still lifes and interiors. Although he travelled to Italy, his first command of landscape was the Italianate landscape of Fano, painted in 1817. In England, he began to concentrate on architecture and marine scenes. The high point of his career came in 1820 when he was invited to join the Royal Academy. His paintings were not auctioned by Christie’s during his lifetime, but Christie’s began to sell his paintings after his death. Egmont died in Antwerp in 1860. Life and work There are very few paintings by Egmont with dates earlier than 1820 and the London Connection probably had to do with his membership in the Royal Academy. His early works are in a broadly established style, with strong emotional content and a preference for elegant compositions of architectural structures. As Egmont moved away from the earlier approach and focused on the architecture of places, the paintings became more subtle. Egmont’s work is held in the Royal Collection, the Metropolitan Museum of Art, the National Gallery of Scotland, the J. Paul Getty Museum and the Yale Center for British Art. He exhibited at the Paris Salon from 1831 to 1839. His paintings were described as “showing us…a mind well versed in the arts, however unpolished”. Egmont claimed to have initially trained under Gijsbreght Leemans in Antwerp, and this influenced his work. Family Egmont was an only child of the artist Egmont Haverman (1671-1743). Egmont Haverman was a portrait painter who joined the Antwerp Guild of Saint Luke in 1704 and became a master in 1713. On 16 September 50b96ab0b6

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