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To clarify the functional role of AP-2 alpha or beta and AP-2 delta in the nervous system, we expressed cDNAs for these transcription factors in PC12 cells and assessed their ability to induce neurite outgrowth. Expression of AP-2 alpha or AP-2 beta alone failed to induce neurite outgrowth. When the cDNAs for both AP-2 alpha and AP-2 beta were co-expressed, an increase in neurite outgrowth was observed, suggesting that the two transcription factors may cooperate to induce this process. To elucidate the mechanism of cooperation, we investigated the activities of extracellular signal-regulated kinases (ERKs) and phosphatidylinositol 3 kinase (PI3K) in PC12 cells transfected with the AP-2 beta-expressing construct. The constitutive and inducible activities of ERKs decreased in parallel with AP-2 beta expression, but those of PI3K did not. These results suggest that AP-2 beta may inhibit neurite outgrowth by
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