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Immunohistochemistry was used in the analysis of recombination events in the central nervous system. An erythropoietin-producing human brain tumor cell line was used as a model system for analysis of genotypic aberrations. Loss of heterozygosity at two loci on chromosome 13 was assessed by microdissection and hybridization with probes for the immunoglobulin heavy chain gene at chromosomal locus q 11.1 and an erythropoietin gene probe at locus p22.7. Immunohistochemistry was applied to the analysis of chromosomes 15 and 17 and for immunolabeling of the erythropoietin produced by the tumor cell line. There was no evidence of a breakpoint at locus q 11.1 or p22.7 on the erythropoietin-producing cells; however, pY815 of the erythropoietin molecule was localized to the perinuclear region of the cell line. Asp395, another erythropoietin epitope, was seen to be associated with the nucleus, in contrast to pY896, an epitope whose localization corresponded to that of pY815. Immunolabeling for the beta chain of the erythropoietin molecule did not reveal a breakpoint at chromosomal locus q 11.1. This study illustrates the usefulness of immunohistochemistry for the study of somatic genetic changes.
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/**
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