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19-Feb-2015 15:43
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This invention relates to the production of branched DNA. A dendrimer, that is a branched molecule that comprises a first generation unit having branches terminating with a first generation unit and a second generation unit having branches terminating with a second generation unit and so forth, is reacted with a polynucleotide template, preferably double-stranded DNA or RNA. Annealed polynucleotide strands (made by the method of the invention) have sequences on their backbones that permit them to be bound to a surface of a substrate, for example, silicon or glass, such that the polynucleotides may be used in microarray and gene sequence analysis. The polynucleotides in turn form a base pair with one or more target polynucleotides to form polynucleotide hybrids.
B. Background of the Invention
The double-stranded polynucleotides are bound to a substrate having single-stranded polynucleotide sequences pre-existing on their backs. The polynucleotides that are bound to the substrate via their backbones are capable of hybridization with nucleic acids in solution or immobilized on the substrate.
The polynucleotides that are hybridized to a target polynucleotide on the substrate are then contacted with a reagent, for example, a fluorescent dye having a fluorescent tag, and/or a radioactive tag having a radioactive tag,
https://colab.research.google.com/drive/1FNE9DACxMduuyqoB_CPsjZ3OxvGmBWPH
https://colab.research.google.com/drive/18yev89Xcuzm7cCkcgz52j6i5gggIYyCJ
https://colab.research.google.com/drive/1F_O6RCbSq2whon6x-ho9QyRcf2u2YllI
https://colab.research.google.com/drive/1Y69LR13iC8vP16SSLMY58nJ8EQAuAFNL
https://colab.research.google.com/drive/1SJLJuzbcBZ8aET2D6UCMu_WF4RPV7gzu
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